VSVG慢病毒感染不同細(xì)胞系和原代細(xì)胞的相對(duì)效率-女人爽到高潮潮噴在線觀看直播了-網(wǎng)絡(luò)春晚2024央視
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| cell type | 相對(duì)感染效率(2) |
| cell lines | ||
| (human) | ||
| hek293 | embryonic kidney cells | ++++ |
| te671 | rhabdomyosarcoma cells | ++++ |
| hela | cervical adenocarcinoma | ++++ |
| hepg2 | hepatoma cells | ++++ |
| ib3-1 | cf bronchial epithelial cells | +++ |
| k562 | chronic myelogenous leukemia | ++++ |
| u937 | myeloid leukemia cells | ++++ |
| a20 | b cell lymphoma | + |
| ebvblasts | ebv transformed b lymphoma | +++ |
| (mouse) | ||
| renca | renal cell carcinoma | ++++ |
| ct26 | colon carcinoma | ++++ |
| nih3t3 | fibroblasts | +++ |
| baf3 | pre-b lymphoma | +++ |
| tf1 | proerythro blastic leukemia cells | ++++ |
| dc2.4 | immortalized immature dc | ++/+++ |
| p19 | embryonic carcinoma | +++ |
| primarycells | ||
| (human) | ||
| huvec | umbilical vein endothelial cells | +++ |
| cd34+cells | hematopoietic stem/progenitor cells | ++/+++ |
| monocytes | myeloid cells | ++ |
| dc | immature dendritic cells | +++ |
| tcells | lymphocytes | +++ |
| bcells | lymphocytes | + |
| (other species) | ||
| esc | mouse embryonic stem cells | +++ |
| neuron | rat neuronal cells | +++ |
| liverstemcells | rat oval cells | +++ |
| liver | primary rat hepatocytes | ++ |
| rpe | rat retinal pigment epithelial cells | ++++ |
| rod | chicken eyerod cells | ++ |
| bonemarrow | mouse bm mixed population | +++ |
| hsc | mouse hematopoietic stem/progenitor cells | ++ |
(1)上表中的原代細(xì)胞感染效率和部分細(xì)胞系感染效率基于不同文獻(xiàn)中的實(shí)驗(yàn)結(jié)果。由于采用了不同報(bào)告基因和載體,感染效率可能有很大差別。表中的感染效率僅供參考。
(2)相對(duì)感染效率定義:++++=moi為1-5時(shí)有大于50%細(xì)胞感染;+++=moi為5-10時(shí)有大于50%細(xì)胞感染;++=moi為10-20時(shí)有大于30%細(xì)胞感染;+=moi大于20時(shí)有1-20%細(xì)胞感染。